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International Journal of Agriculture, Environment and Biotechnology
Year : 2018, Volume : 11, Issue : 3
First page : ( 589) Last page : ( 599)
Print ISSN : 0974-1712. Online ISSN : 2230-732X.
Article DOI : 10.30954/0974-1712.06.2018.21

Effects of Pre-Sowing seed treatment on the growth rate of seedlings and the activity of the excretory system of the wheat root in aquatic culture

Azeez Zaid, Kovalova Marina*, Bozhkov Anatoly

Biology research institute of V. N. Karazin Kharkov National University, sg. Svobody, 4, 61007, Kharkov, Ukraine

*Corresponding author: bozhkov@univer.kharkov.ua

Online published on 27 September, 2018.

Abstract

The qualitative and quantitative composition of the rhizosphere is known to be the result of the metabolic activity of the root and it regulates the functional activity of the root. Therefore, it works as autoregulation in the root activity. The study of the system of autoregulation is actually not only for the physiology of plants, but also for modern biotechnologies. The contents of proteins, amino acids and total carbohydrates were determined during the formation of the rhizosphere of the wheat's roots from 1 to 3 day of growth in aquatic culture. In addition to that, the role of microorganisms in the formation of the root microenvironment with different models of pre-sowing seed treatment was investigated. It was found that, the pre-sowing seed treatment affects the qualitative and quantitative composition of root exudates. The pre-sowing treatment of seeds with potassium permanganate increased the protein content in the composition of exudates in the 1st day of seedling about 75%, but it decreased in the 2nd day about 40% if compared with the control variant. The pre-sowing treatment of seeds with ethyl alcohol and sodium hypochlorite reduced the protein content of exudates in the 2nd day of seedlings about 45% and did not affect this indicator in 1 and 3rd day of seedlings in comparison with the control variant. The pre-sowing treatment with potassium permanganate insignificantly affected the mount of microorganisms, while the pre-sowing treatment with alcohol and sodium hypochlorite reduced the amount of microorganisms in the root exudates two times if compared with the control variant.

Highlights

  • The microbiota which seen on the grains did not have significant effect on the qualitative and quantitative composition of the root exudates.

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Keywords

Wheat seedlings, root exudates, the microbiota, pre-sowing treatment.

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