Email Alert RSS
Volume 14 Issue 4
Jul.  2023
Turn off MathJax
Article Contents
Abstract
References
Related Articles
ZHENG Meiling, FAN Qingli, ZHONG Lianmei. Intestinal Microbiota Polymorphism of Epilepsy Patients in Middle and High Altitude Areas: A Case-control Study[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(4): 881-888. DOI: 10.12290/xhyxzz.2022-0533
Citation: ZHENG Meiling, FAN Qingli, ZHONG Lianmei. Intestinal Microbiota Polymorphism of Epilepsy Patients in Middle and High Altitude Areas: A Case-control Study[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(4): 881-888. DOI: 10.12290/xhyxzz.2022-0533
shu

Intestinal Microbiota Polymorphism of Epilepsy Patients in Middle and High Altitude Areas: A Case-control Study

  • 1.

    Department of Graduate School, Qinghai University, Xining 810016, China

  • 2.

    Department of Neurology, Qinghai Provincial People's Hospital, Xining 810000, China

  • 3.

    Department of Neurology, The First Affiliated Hospital of Kunming Medical University, 650000, China

Funds: 

Qinghai Science and Technology Department Project 2022-ZJ-761

More Information
  • Corresponding author:

    FAN Qingli, E-mail: 1628018509@qq.com

  • Received Date: September 22, 2022
  • Accepted Date: January 02, 2023
  • Issue Publish Date: July 29, 2023
    Graphical Abstract
    • Abstract
  •   Objective  To investigate the composition and distribution characteristics of intestinal flora in patients with epilepsy at middle-high altitude, in order to provide theoretical reference for the etiological study of epilepsy.
      Methods  From September 2021 to August 2022, we continuously recruited middle-high altitude epilepsy (MHE) patients (altitude 2200-4500 m) and healthy people at the same altitude as middle- high altitude normal (MHN). MHE were epileptic patients in neurology department of Qinghai Provincial People's Hospital, and MHN were healthy people in physical examination center of Qinghai Provincial People's Hospital which matched 1∶1 with MHE by age and gender. Stool tissue specimens from both groups were collected, and the 16S rDNA V3 to V4 regions of the intestinal flora were sequenced for DNA and analyzed bioinformatically.
      Results  A total of 32 cases of MHE and 33 cases of MHN who met the inclusion and exclusion criteria. Alfa diversity analysis showed that ACE index, Shannon index and Chao index in MHE group were lower than those in MHN group (all P < 0.05). Beta diversity analysis showed that there were significant differences in the distribution of intestinal flora between the two groups. In the analysis of intestinal flora composition, MHE group also showed different characteristics from MHN group at phylum level and genus level. The relative abundance of Verrucomicrobiota, Collinsella, Fusobacteriota, Bifidobacterium and other bacteria increased, whereas the relative abundance of Bacteroidota, Bacteroides, Prevotella, Faecalibacterium and other bacteria decreased.
      Conclusion  The intestinal flora of epilepsy patients at middle and high altitude has different polymorphisms from that of healthy people at the same altitude, which may be associated with epilepsy.
    • FullText(HTML)
    • References (30)
  • [1]
    Iannone LF, Preda A, Blottière HM, et al. Microbiota-gut brain axis involvement in neuropsychiatric disorders[J]. Expert Rev Neurother, 2019, 19: 1037-1050. DOI: 10.1080/14737175.2019.1638763
    [2]
    Villafuerte FC, Corante N. Chronic mountain sickness: clinical aspects, etiology, management, and treatment[J]. High Alt Med Biol, 2016, 17: 61-69. DOI: 10.1089/ham.2016.0031
    [3]
    Mingji C, Onakpoya IJ, Perera R, et al. Relationship between altitude and the prevalence of hypertension in Tibet: a systematic review[J]. Heart, 2015, 101: 1054-1060. DOI: 10.1136/heartjnl-2014-307158
    [4]
    常琳, 王小姗. 中国癫痫流行病学调查研究进展[J]. 国际神经病学神经外科学杂志, 2012, 39: 161-164. https://www.cnki.com.cn/Article/CJFDTOTAL-GWSK201202016.htm
    [5]
    Fisher RS, Acevedo C, Arzimanoglou A, et al. ILAE official report: a practical clinical definition of epilepsy[J]. Epilepsia, 2014, 55: 475-482. DOI: 10.1111/epi.12550
    [6]
    Safak B, Altunan B, Topcu B, et al. The gut microbiome in epilepsy[J]. Microb Pathog, 2020, 139: 103853. DOI: 10.1016/j.micpath.2019.103853
    [7]
    Wang HX, Wang YP. Gut Microbiota-brain Axis[J]. Chin Med J (Engl), 2016, 129: 2373-2380. DOI: 10.4103/0366-6999.190667
    [8]
    Thursby E, Juge N. Introduction to the human gut microbiota[J]. Biochem J, 2017, 474: 1823-1836. DOI: 10.1042/BCJ20160510
    [9]
    Peng A, Qiu X, Lai W, et al. Altered composition of the gut microbiome in patients with drug-resistant epilepsy[J]. Epilepsy Res, 2018, 147: 102-107. DOI: 10.1016/j.eplepsyres.2018.09.013
    [10]
    Dahlin M, Prast-Nielsen S. The gut microbiome and epilepsy[J]. EBioMedicine, 2019, 44: 741-746. DOI: 10.1016/j.ebiom.2019.05.024
    [11]
    王世荣, 岳寿松. 微生态制剂和肠道菌群与亚健康[J]. 中国微生态学杂志, 2017, 29: 370-373. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGWS201703030.htm
    [12]
    Holmes M, Flaminio Z, Vardhan M, et al. Cross talk between drug-resistant epilepsy and the gut microbiome[J]. Epilepsia, 2020, 61: 2619-2628. DOI: 10.1111/epi.16744
    [13]
    He Z, Cui BT, Zhang T, et al. Fecal microbiota transplantation cured epilepsy in a case with Crohn's disease: The first report[J]. World J Gastroenterol, 2017, 23: 3565-3568. DOI: 10.3748/wjg.v23.i19.3565
    [14]
    Rothschild D, Weissbrod O, Barkan E, et al. Environment dominates over host genetics in shaping human gut microbiota[J]. Nature, 2018, 555: 210-215. DOI: 10.1038/nature25973
    [15]
    Li L, Zhao X. Comparative analyses of fecal microbiota in Tibetan and Chinese Han living at low or high altitude by barcoded 454 pyrosequencing[J]. Sci Rep, 2015, 5: 14682. DOI: 10.1038/srep14682
    [16]
    Gong X, Liu X, Chen C, et al. Alteration of gut microbiota in patients with epilepsy and the potential index as a biomarker[J]. Front Microbiol, 2020, 11: 517797. DOI: 10.3389/fmicb.2020.517797
    [17]
    Cui G, Liu S, Liu Z, et al. Gut microbiome distinguishes patients with epilepsy from healthy individuals[J]. Front Microbiol, 2021, 12: 696632.
    [18]
    de la Cuesta-Zuluaga J, Mueller NT, Corrales-Agudelo V, et al. Metformin is associated with higher relative abundance of mucin-degrading akkermansia muciniphila and several short-chain fatty acid-producing microbiota in the gut[J]. Diabetes Care, 2017, 40: 54-62. DOI: 10.2337/dc16-1324
    [19]
    Roggenbuck M, Bærholm Schnell I, Blom N, et al. The microbiome of New World vultures[J]. Nat Commun, 2014, 5: 5498. DOI: 10.1038/ncomms6498
    [20]
    Thambi M, Nathan J, Radhakrishnan K. Can change in gut microbiota composition be used as a surrogate marker of treatment efficacy of ketogenic diet in patients with drug-resistant epilepsy?[J]. Epilepsy Behav, 2020, 113: 107444. DOI: 10.1016/j.yebeh.2020.107444
    [21]
    Yang Y, Weng W, Peng J, et al. Fusobacterium nucleatum increases proliferation of colorectal cancer cells and tumor development in mice by activating Toll-like receptor 4 signaling to nuclear factor-κB, and up-regulating expression of microRNA-21[J]. Gastroenterology, 2017, 152: 851-866. DOI: 10.1053/j.gastro.2016.11.018
    [22]
    Galic MA, Riazi K, Pittman QJ. Cytokines and brain excitability. [J]. Front Neuroendocrinol, 2012, 33: 116-125. DOI: 10.1016/j.yfrne.2011.12.002
    [23]
    Jiang W, Wu N, Wang X, et al. Dysbiosis gut microbiota associated with inflammation and impaired mucosal immune function in intestine of humans with non-alcoholic fatty liver disease[J]. Sci Rep, 2015, 5: 8096. DOI: 10.1038/srep08096
    [24]
    Chen J, Wright K, Davis JM, et al. An expansion of rare lineage intestinal microbes characterizes rheumatoid arthritis[J]. Genome Med, 2016, 8: 43. DOI: 10.1186/s13073-016-0299-7
    [25]
    Mao LY, Ding J, Peng WF, et al. Interictal interleukin-17A levels are elevated and correlate with seizure severity of epilepsy patients[J]. Epilepsia, 2013, 54: e142-e145. DOI: 10.1111/epi.12337
    [26]
    Rana A, Musto AE. The role of inflammation in the develop-ment of epilepsy[J]. J Neuroinflammation, 2018, 15: 144. DOI: 10.1186/s12974-018-1192-7
    [27]
    Ang QY, Alexander M, Newman JC, et al. Ketogenic diets alter the gut microbiome resulting in decreased intestinal Th17 cells. [J]. Cell, 2020, 181: 1263-1275. DOI: 10.1016/j.cell.2020.04.027
    [28]
    Olson CA, Lum GR, Hsiao EY. Ketone bodies exert ester-ordinary suppression of bifidobacteria and Th17 cells[J]. Cell Metab, 2020, 31: 1049-1051. DOI: 10.1016/j.cmet.2020.05.011
    [29]
    Maslowski KM, Vieira AT, Ng A, et al. Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43[J]. Nature, 2009, 461: 1282-1286. DOI: 10.1038/nature08530
    [30]
    Joseph J, Depp C, Shih PB, et al. Modified mediterranean diet for enrichment of short chain fatty acids: potential adjunctive therapeutic to target immune and metabolic dysfunction in schizophrenia?[J]. Front Neurosci, 2017, 11: 155.
    • Related Articles

  • Related Articles

    [1]DUAN Hanyu, ZHU Qianmei, Cirendeji, LI Yi, MIAO Xuhan, ZHANG Yuelun, LIU Zijia, Labaciren, SHEN Le, HUANG Yuguang. Risk Factors of Hypoxemia During Non-cardiothoracic Surgery for Populations at High Altitude in Tibet: A Prospective Cohort Study[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(3): 646-651. DOI: 10.12290/xhyxzz.2022-0610
    [2]ZHAO Lidan, MENG Xia, XU Haojie, ZHANG Fengchun. Prospect of Gut Microbiota-based Intervention in Autoimmune Disease Control[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(5): 740-746. DOI: 10.12290/xhyxzz.2022-0245
    [3]GAO Zhongshan, REN Ming, MA Yulan, ZHU Lulu. Polymorphism of Gut Microbiota in High Altitude Tibetan Patients with Coronary Artery Heart Disease[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(2): 332-340. DOI: 10.12290/xhyxzz.2021-0652
    [4]WANG Pei, MA Liangkun, LIU Juntao. Difference in Gut Microbiota between the First and the Second Trimester of Pregnancy and the Association of Gut Microbiota with Gestational Diabetes Mellitus: A Prospective Cohort Study[J]. Medical Journal of Peking Union Medical College Hospital, 2021, 12(5): 721-728. DOI: 10.12290/xhyxzz.20200122
    [5]Bei-di CHEN, Li-dan ZHAO, Xuan ZHANG. Gut Microbiota and Systemic Lupus Erythematosus[J]. Medical Journal of Peking Union Medical College Hospital, 2020, 11(3): 258-263. DOI: 10.3969/j.issn.1674-9081.20200056
    [6]Ri-liga WU, Rong MU. Intestinal Microbiota in Systemic Sclerosis[J]. Medical Journal of Peking Union Medical College Hospital, 2019, 10(3): 263-267. DOI: 10.3969/j.issn.1674-9081.2019.03.013
    [7]Yu-hao JIAO, Bei-di CHEN, Xuan ZHANG. Interplay between the Gut Microbiota and the Innate Immune System[J]. Medical Journal of Peking Union Medical College Hospital, 2019, 10(3): 257-262. DOI: 10.3969/j.issn.1674-9081.2019.03.012
    [8]Li-wei WANG, Zhi-min DUAN, Jian-bo TONG, Rong ZENG, Hao-xiang XU, Min LI. Diversity Analysis of Intestinal Microbiota in Psoriasis Patients: A Single-center Prospective Study[J]. Medical Journal of Peking Union Medical College Hospital, 2019, 10(3): 223-230. DOI: 10.3969/j.issn.1674-9081.2019.03.007
    [9]Ying-he LI, Xiang-chen MENG, Peng-guang YAN, Hong YANG, Ji LI, Jia-ming QIAN, Jing-nan LI. Relevance between Glucocorticoid Treatment and the Intestinal Microbiota of Patients with Moderately Severe Ulcerative Colitis: A Single-center Retrospective Study[J]. Medical Journal of Peking Union Medical College Hospital, 2019, 10(3): 216-222. DOI: 10.3969/j.issn.1674-9081.2019.03.006
    [10]Yu-hao JIAO, Feng-chun ZHANG. New Insights into Research on the Role of Gut Microbiota in Human Immune System[J]. Medical Journal of Peking Union Medical College Hospital, 2019, 10(3): 193-196. DOI: 10.3969/j.issn.1674-9081.2019.03.001

  • Cited by

    Periodical cited type(28)

    1. 李妍，韩彤昕，毛华伟. 线上与线下医学人文教学结合应用的研究与探索. 中国继续医学教育. 2025(01): 185-188 .
    2. 李研，赵琳琳. 英美医学教育中戏剧元素的人文透视. 中国医学伦理学. 2025(03): 385-391 .
    3. 张慧颖，王锦帆，马宇昊，何源. 新时代中国医学人文研究热点及趋势探析. 中国医学伦理学. 2025(03): 336-345 .
    4. 王红宇，刘金强，丁俊杰，王红，孟志剑，唐聚花，张思森. 医患沟通技能培训在急重症医学住院医生规培中的应用. 中国现代医生. 2024(02): 74-76 .
    5. 向琳，王浩辰，黄天宇，何鲲. 基于“医教研德”复合型人才培养模式口腔种植学教学改革思考与探索. 中国医药导报. 2024(04): 70-73 .
    6. 俞婧，王瑞，武云. 基于VOSviewer的我国叙事医学相关文献的可视化分析及启示. 中国毕业后医学教育. 2024(03): 207-213 .
    7. 余航，安琪，金李，吴远，陈涛，肖懿慧. BOPPPS教学模式融合课程思政在本科生内科学见习中的应用. 医学教育研究与实践. 2024(03): 349-354 .
    8. 周殷华，程瑜，崔昌杰，田晓辉，符隆文，方婵，廖晓星，陈起坤. 医文融合视域下临床医学人文教学体系研究. 中国医学伦理学. 2024(07): 860-866 .
    9. 王京，贺欢欣，董智瑞，董健，赵明东. 住培医师人文素养的培育. 中国继续医学教育. 2024(20): 186-189 .
    10. 敖玲敏，沈菊. 中华优秀传统文化融入大学生心理健康教育的价值之维与实践之路. 黑龙江高教研究. 2024(12): 120-126 .
    11. 王倩，巩红，刘昌，李雁，李研，辛娟，张月浪. 以临床人文岗位胜任力为导向的医学人文多元教育渠道的探索. 医学教育研究与实践. 2023(01): 68-72 .
    12. 徐燕玲，顾漪. 临床医师医学人文认知现状调查. 中国现代医生. 2023(05): 135-138 .
    13. 冯悦，庞维，郑丽，李胜军，孙逊，冯辉. 新医科和新文科交叉融合对免疫学创新人才培养的探索性改革. 中国免疫学杂志. 2023(06): 1203-1206 .
    14. 柳舟，张亮，王璐，朱睿瑶，王慧娟，张静，雷佳羲，詹丽英. 重症医学科医学人文素质教育的改革与实践. 中国继续医学教育. 2023(12): 10-14 .
    15. 阳磊，彭蓓，张婉丽. 口腔科住院医师规范化培训医学人文教育改革实践. 经济研究导刊. 2023(12): 123-125 .
    16. 赵娟，徐斌. 进修医师自身免疫性肝炎临床带教体会. 继续医学教育. 2023(06): 129-132 .
    17. 刘琴，何自强，骆佳佳，冯凯娜. 以提升人文关怀能力为导向的护理学导论教学模式应用研究. 沈阳医学院学报. 2023(05): 540-543 .
    18. 谢佳君，何勇涛，黄国琼，秦宇彤，王方芳，栗昕，黄春基. 立德树人背景下医学生德育素质培养研究. 中国社会医学杂志. 2023(05): 540-543 .
    19. 吕晓龙，严旭，章涵. 以强化人文素养为导向的全科医学生叙事医学课程模式构建. 济源职业技术学院学报. 2023(04): 33-37 .
    20. 吕少春，宋汉君，栾海艳，何穆涵. 基于医学模拟教学的医学生临床能力培养研究. 医学教育研究与实践. 2022(03): 294-297 .
    21. 李超. 培养高素质临床医生路径分析. 继续医学教育. 2022(04): 61-64 .
    22. 冯矗，陈飞，张树霞，刘古月，李晓宇. 医学人文关怀教育融入外科实习的实践探索. 中医药管理杂志. 2022(11): 88-90 .
    23. 高铭，韩涛，池靖涵，杜振兰，杨常栓，侯豫，花少栋. 创新互动式医学人文教育在儿科住院医师规范化培训中的作用研究. 中国医学伦理学. 2022(07): 806-810 .
    24. 胡艳超，王洪涛，王怡雯，周戬平. 医学人文教育在心血管内科混合式教学中的应用. 医学教育研究与实践. 2022(05): 648-652 .
    25. 叶榆莹. 英语电影字幕翻译对医学生人文教育的作用探究. 才智. 2022(33): 62-65 .
    26. 陶庆才. 抗疫精神融入医学院校课程思政的研究与实践. 浙江医学教育. 2022(05): 268-270+299 .
    27. 张宏伟，熊梓彤，林芷伊. 英国医学人文关怀及其医学教育启示. 农垦医学. 2022(06): 561-564 .
    28. 祝贺，敖俊红，杨蓉娅，李海涛. 全媒体时代医学摄影的边界教育在皮肤病与性病科临床教学中的意义. 实用皮肤病学杂志. 2022(06): 367-369 .

    Other cited types(6)

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (319) PDF downloads (51) Cited by(34)
    Related
    Links: National Health Commission of the People's Republic of China Chinese Academy of Medical Sciences(CAMS) AMLC Check CNKI Database CNKI Term Online Chinese General Practice
    Address：Room 302,Third Floor,Building 7, No. 1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing 100730, China
    Tel：010-69154261/4262
    E - mail：medj@pumch.cn mjpumch@126.com
    Copyright of website：Editorial Office of Medical Journal of Peking Union Medical College Hospital

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return
    x Close Forever Close