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Volume 14 Issue 3
May  2023
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WEI Jiaojiao, LIU Shiwei, DUAN Ruixue, LI Nan, WANG Jiangna. Effects of Vaspin on Pancreatic Beta Cell Function in Type 2 Diabetic Rats by AMPK/mTOR Autophagy Signaling Pathway[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(3): 543-552. DOI: 10.12290/xhyxzz.2022-0183
Citation: WEI Jiaojiao, LIU Shiwei, DUAN Ruixue, LI Nan, WANG Jiangna. Effects of Vaspin on Pancreatic Beta Cell Function in Type 2 Diabetic Rats by AMPK/mTOR Autophagy Signaling Pathway[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(3): 543-552. DOI: 10.12290/xhyxzz.2022-0183
shu

Effects of Vaspin on Pancreatic Beta Cell Function in Type 2 Diabetic Rats by AMPK/mTOR Autophagy Signaling Pathway

  • 1.

    The Ninth Clinical Medical College of Shanxi Medical University, Taiyuan 030009, China

  • 2.

    Department of Endocrinology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, the Third Hospital of Shanxi Medical University, Taiyuan 030032, China

  • 3.

    Department of Endocrinology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

  • 4.

    Department of Graduate School, Shanxi Medical University, Taiyuan 030001, China

Funds: 

Key Project of Research and Development Plan in Shanxi Province 201803D31133

Research Project Supported by Shanxi Scholarship Council of China 2020-180

Fund Program for the Scientific Activities of Selected Returned Overseas Professionals in Shanxi Province 20200032

China Diabetes Young Scientific Talent Research Project Application Form 2018-N-01-28

More Information
  • Corresponding author:

    LIU Shiwei, E-mail: lswspring6@aliyun.com

  • Received Date: April 05, 2022
  • Accepted Date: May 16, 2022
  • Issue Publish Date: May 29, 2023
    Graphical Abstract
    • Abstract
  •   Objective  To investigate the mechanism of Vaspin in improving the pancreatic beta cell function in type 2 diabetic (T2DM) rats.
      Methods  The diabetic rat model was established by feeding high-fat and high-sugar diet combined with intraperitoneal injection of streptozotocin. T2DM rats were randomly divided into T2DM group(n=10) and Vaspin group(n=10), with the same age SD rats fed with normal chow as normal control group (n=10). Before modeling, before Vaspin intervention, at 4 weeks and 8 weeks Vaspin intervention, the level of body weight and fasting blood-glucose(FBG) of rats were recorded. At 8 weeks of Vaspin intervention, fasting insulin (FINS), glucose tolerance and insulin sensitivity, pancreatic β-cell function and autophagy-related protein expression levels were measured in three groups of rats, and histopathological morphology of the pancreas was observed.
      Results  Before Vaspin intervention, at the 4 weeks and 8 weeks Vaspin intervention, compared with the normal control group, the level of body weight decreased and FBG increased in T2DM group and Vaspin group(all P < 0.05). At 8 weeks Vaspin intervention, compared with T2DM group, the level of body weight increased and FBG decreased in Vaspin group (all P < 0.05). Histopathology showed that the pancreatic tissue was normal, islet cells were arranged evenly, neatly and regularly in normal control group. In T2DM group, the islet structure was obviously destroyed, and the cells were unevenly distributed and irregularly shaped. Compared with T2DM group, the structural damage and morphological damage of islet cells in Vaspin group were significantly reduced. At the 8 weeks intervention, compared with the normal control group, the level of FINS decreased, while the area under the blood glucose curve of intraperitoneal glucose tolerance test(IPGTT) and intraperitoneal insulin tolerance test(IPITT) increased in T2DM group and Vaspin group(all P < 0.05). Compared with T2DM group, the level of FINS increased, while the area under blood glucose curve of IPGTT and IPITT decreased in Vaspin group(all P < 0.05). At the 8 weeks intervention, the high glucose clamp test showed that the glucose infusion rate, the level of insulin secretion in the first phase and the second phase in Vaspin group were lower than those in the normal control group, but all indexes in Vaspin group were higher than those in T2DM group (all P < 0.05). At the 8 weeks intervention, immunohistochemical and Western blot results showed that compared with the normal control group, the level of insulin expression and the ratio of p-mTOR/mTOR in pancreatic tissue of rats in T2DM group decreased, while the protein levels of P62, microtubule associated protein 1 light chain 3 (LC3), p-AMPK/AMPK ratio and LC3 Ⅱ/LC3 Ⅰ ratio increased. Compared with T2DM group, the levels of insulin, LC3 protein, p-AMPK/AMPK ratio and LC3 Ⅱ/LC3 Ⅰ ratio in pancreatic tissue of rats in Vaspin group increased, while p-mTOR/mTOR ratio and P62 protein expression decreased (all P < 0.05).
      Conclusion  Vaspin can enhance the autophagy of pancreatic beta cells, improve pancreatic beta cells function by AMPK/mTOR signaling pathway in the type 2 diabetic rats.
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    • References (28)
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